高粱苗期耐盐碱性QTL定位

2023-03-13 11:36:14 第一文档网 [ 字体：小中大 ] [ 阅读： ] [

【#第一文档网# 导语】以下是®第一文档网的小编为您整理的《高粱苗期耐盐碱性QTL定位》，欢迎阅读！
苗期,高粱,碱性,定位,QTL
高粱苗期耐盐碱性QTL定位

唐朝臣;高建明;韩芸;罗峰;裴忠有;孙守钧

【期刊名称】《华北农学报》【年(卷),期】2015(000)003

【摘要】为定位混合盐碱胁迫下调控高粱苗期性状的QTL位点，发掘耐盐碱基因位点及其紧密连锁标记。以耐盐碱和盐碱敏感组合BJ-299×Tx622 B构建的F2群体为材料，采用SSR标记技术和区间作图法对170个F2∶3家系进行高粱苗期相对存活率（ RL）、相对干质量（ RDW）、相对鲜质量（ RFW）和相对苗高（ RSH）的QTL分析。结果表明，试验材料耐盐碱性状符合数量遗传的特点，共定位到3个QTL位点（ qSAT-A、qSAT-D和qSAT-J ）调控高粱苗期耐盐碱性状，分布在 A、D 和J 染色体上，位于标记 Sam27281～Sam22486、

Sam11433～Sam78379和Sam62346 a ～Sam38392。qSAT-A和qSAT-D同时调控RL、RDW、RFW和RSH性状，表型贡献率分别为14．3％，9.7％，8．0％，10．6％和8．7％，13．5％，18．8％，13．6％；qSAT-J位点调控RL性状，贡献率为7．3％。 qSAT-A和qSAT-D为调控高粱苗期耐盐碱性状的重要位点，有望在高粱耐盐碱性状分子标记辅助育种发挥重要作用。%Aimed to identify QTLs associated with seedling four traits under complex salt-alkaline stress in Sor-ghum and provide target loci and linked molecular markers for marker assisted selection of Sorghum salt -alkaline tolerant-related traits,an F2 population with 170 lines derived from cross between BJ-299 ×Tx622B were used in this study.Quantitative trait loci (QTLs) for relative livability (RL),relative dry weight (RDW),relative fresh weight (RFW)

and relative seedling height (RSH) at seedling stage were analyzed by interval mapping method . The results demonstrated that salt-alkaline tolerance at seedling stage of sorghum was a complex quantitative trait and controlled by multiple genes .Correlation coefficients between each two traits in the F 2∶3 populations under com-plex salt-alkaline stress condition showed significant positive correlation .A total of 3 QTLs ( qSAT-A,qSAT-D and qSAT-J) located on three major chromosome regions ( Sam27281-Sam22486,Sam11433-Sam78379 and Sam62346a-Sam50778) on chromosome A,D and J,respectively.The negative effects were all from the salt-alkaline sensitive parent Tx622 B.qSAT-A and qSAT-D were controlling four salt-alkaline tolerant related traits simultaneously , and explained phenotypic variation ranging from 8 .0% to 18 .8%.QTL qSAT-J for RL was detected with phenotypic variation explained of 7.3%.The two major QTLs (qSAT-A,qSAT-D) and three chromosome regions played crucial role in salt-alkaline tolerance of sorghum , which may have a potential in the practice of marker assisted sorghum breeding for improving salt-alkaline tolerance . 【总页数】6页(P42-47)

【作者】唐朝臣;高建明;韩芸;罗峰;裴忠有;孙守钧

【作者单位】天津农学院农学与资源环境学院，天津 300384;天津农学院农学与资源环境学院，天津 300384;天津农学院农学与资源环境学院，天津 300384;天津农学院农学与资源环境学院，天津 300384;天津农学院农学与资源环境学院，天津 300384;天津农学院农学与资源环境学院，天津 300384

【正文语种】中文【中图分类】S514.03 【相关文献】

1.高丹草(高粱×苏丹草)产量及其构成因素的QTL定位与分析2.高粱抗蚜QTL定位3.高粱株高性状的QTL定位初步分析4.棉花耐盐碱性状的QTL定位5.高粱F_6代群体分蘖数的QTL定位

因版权原因，仅展示原文概要，查看原文内容请购买