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抗衰老保健食品中白藜芦醇和辅酶Q_(10)的含量测定
姜伟;杨小妹;陆豫;余勃
【期刊名称】《广州化工》 【年(卷),期】2012(040)010
【摘 要】建立了同时测定抗衰老保健食品中白藜芦醇和辅酶Q10的含量的HPLC方法。采用Welch materials C18液相色谱柱,甲醇∶异丙醇(55∶45)为流动相,流速设定为1 mL·min^-1,检测波长为PDA定时波长(0.00,306.0)(6.00,306.0)(6.01,275.0)(20.00,275.0),实现了白藜芦醇和辅酶Q10的良好分离分析。白藜芦醇的线性范围为0.52~156.00μg·mL^-1,r=0.9999;辅酶Q10的线性范围为0.49~195.52μg.mL^-1,r=0.9999。平均加标回收率及RSD分别为97.70%(RSD 0.6%)和97.40%(RSD 0.4%)。本方法灵敏度高、选择性好、操作简单,可方便地用于检测抗衰老类保健食品中白藜芦醇和辅酶Q10的含量。%A high performance liquid chromatographic(HPLC) method was established for the simultaneous determination of resveratrol and Coenzyme Q10 in anti-aging health-care food.The determination of resveratrol and Coenzyme Q10 by HPLC was performed with Welch materials ODS C18 and the mobile phase of methanol:isopropanol(55:45) at a flow rate of 1.0 mL·min^-1,and the detection wavelength was conducted at(0.00,306.0),(6.00,306.0),(6.01,275.0),and(20.00,275.0).Results showed that a good separation of resveratrol and Coenzyme Q10 was observed.The calibration curve of resveratrol was linear within the range of 0.52~156.00 μg·mL-1 and r=0.9999.The calibration curve of Coenzyme Q10 was linear
within the range of 0.49~195.52 μg·mL^-1 and r=0.9999.The average recovery rate of resveratrol was 97.70% and the RSD was 0.6%.The average recovery rate of Coenzyme Q10 was 97.40% and the RSD was 0.4%.The HPLC method was characterized by high sensitivity,good selectivity,simple operation of determination of resveratrol and Coenzyme Q10 in anti-aging health-care food.
【总页数】2页(P121-122) 【作 者】姜伟;杨小妹;陆豫;余勃
【作者单位】南昌市食品药品检验所,江西南昌330038;深圳市蛇口人民医院,广东深圳518067;南昌大学中德联合研究院,江西南昌330038;南昌大学中德联合研究院,江西南昌330038 【正文语种】中 文 【中图分类】TS207 【相关文献】
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